Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety and Ef

0
322

2015, pp 113-123

Date: 30 Sep 2014

Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety and Efficacy Evaluation

 

Abstract

Non-expanded adipose tissue-derived stem cells (ADSCs) are commonly used in preclinical and clinical articular cartilage injury treatment; however, there are usually insufficient non-expanded ADSCs for transplantation. This research aims to evaluate the safety and efficacy of expanded ADSC transplantation in a mouse model. The stromal vascular fraction from abdominal adipose tissue was subcultured for ten passages to enrich for and expand the number of ADSCs. The safety of expanded ADSCs was assessed by evaluating their “stemness” via ADSC-specific marker expression, the expression of two tumorigenesis-related genes (Oct3/4 and Nanog) using real-time RT-PCR, and in vivo tumor formation in NOD/SCID mice. ADSC efficacy was determined by assessing their chondrocytic differentiative potential in vitro in cartilage-inducing medium as well as in vivo via injection into a NOD/SCID mice joint failure model. The results showed that expanded ADSCs were negative for Oct3/4 and Nanog and did not induce tumor formation in mice. Furthermore, ADSCs differentiated into chondrocytes, both in vitro and in vivo, and enhanced the regeneration of articular cartilage in NOD/SCID mice as compared with the control. These results confirm that expanded ADSCs are safe and effective for the treatment of injured articular cartilage and offer a promising therapy for degenerative cartilaginous diseases.

 
 
 
Title
Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety and Efficacy Evaluation
Book Title
Regenerative Medicine
Book Subtitle
Using Non-Fetal Sources of Stem Cells
Book Part
Part III
Pages
pp 113-123
DOI
10.1007/978-1-4471-6542-2_11
Print ISBN
978-1-4471-6541-5
Online ISBN
978-1-4471-6542-2
Publisher
Springer London
Additional Links
Topics
Industry Sectors
eBook Packages
Editors
Editor Affiliations
  • 1. Calcutta School of Tropical Medicine
  • 2. Boston University School of Medicine
Authors
Author Affiliations
  • 3. Laboratory of Stem Cell Research and Application, University of Science¸ Vietnam National University, Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, 08000, Vietnam
  • 4. Orthopaedic Department, University Medical Center, Ho Chi Minh City, University of Medicine and Pharmacology, Ho Chi Minh City, 215- 217 Hong Bang, District 5, Ho Chi Minh City, 08000, Vietnam
  • 5. Department of Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, 217 Hồng Bàng, 11th Ward, District 5, Ho Chi Minh City, 08000, Vietnam